pbr buffer Search Results


96
Valiant Co Ltd buffer
Buffer, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ProSci Incorporated pbs
Confocal micrographs of HSV-infected neurons stained for gC antigen at 15 hpi and after addition of human anti-gD monoclonal antibody (top) or control medium (bottom). The HSV inoculum (5 TCID50/cell) was aspirated after 1 h of incubation, and the cells were carefully washed once with HBSS. The HSV-infected or mock-infected dissociated neuronal cultures, incubated with a 1:2,500 dilution (400 ng/ml) of human anti-gD antibody, were fixed in 2.5% formaldehyde (ProSci Tech) in Sorensons buffer (pH 7.4) for 30 min <t>and</t> <t>permeabilized</t> with 0.1% Triton X-100 (Sigma) in <t>PBS</t> for 20 min. Nonspecific staining was blocked by incubation with 5% mouse serum in HBSS for 15 min. The cells on coverslips were then incubated with fluorescein isothiocyanate-conjugated anti-gC1 antibody (Syva Microtrak) (1:100 dilution), rinsed three times with HBSS, and mounted in mounting fluid (Syva Microtrak). Stained neurons were examined with a Bio-Rad MRC 600 confocal microscope. Note the similar distributions of gC antigen in the axon and cytoplasm in both micrographs. Bars, 40 μm (top) and 20 μm (bottom).
Pbs, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cambridge Isotope Laboratories trimethylsilyl propionicd4-sodium salt tsp-d4
Confocal micrographs of HSV-infected neurons stained for gC antigen at 15 hpi and after addition of human anti-gD monoclonal antibody (top) or control medium (bottom). The HSV inoculum (5 TCID50/cell) was aspirated after 1 h of incubation, and the cells were carefully washed once with HBSS. The HSV-infected or mock-infected dissociated neuronal cultures, incubated with a 1:2,500 dilution (400 ng/ml) of human anti-gD antibody, were fixed in 2.5% formaldehyde (ProSci Tech) in Sorensons buffer (pH 7.4) for 30 min <t>and</t> <t>permeabilized</t> with 0.1% Triton X-100 (Sigma) in <t>PBS</t> for 20 min. Nonspecific staining was blocked by incubation with 5% mouse serum in HBSS for 15 min. The cells on coverslips were then incubated with fluorescein isothiocyanate-conjugated anti-gC1 antibody (Syva Microtrak) (1:100 dilution), rinsed three times with HBSS, and mounted in mounting fluid (Syva Microtrak). Stained neurons were examined with a Bio-Rad MRC 600 confocal microscope. Note the similar distributions of gC antigen in the axon and cytoplasm in both micrographs. Bars, 40 μm (top) and 20 μm (bottom).
Trimethylsilyl Propionicd4 Sodium Salt Tsp D4, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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93
R&D Systems thrombospondin 1 tsp 1
Confocal micrographs of HSV-infected neurons stained for gC antigen at 15 hpi and after addition of human anti-gD monoclonal antibody (top) or control medium (bottom). The HSV inoculum (5 TCID50/cell) was aspirated after 1 h of incubation, and the cells were carefully washed once with HBSS. The HSV-infected or mock-infected dissociated neuronal cultures, incubated with a 1:2,500 dilution (400 ng/ml) of human anti-gD antibody, were fixed in 2.5% formaldehyde (ProSci Tech) in Sorensons buffer (pH 7.4) for 30 min <t>and</t> <t>permeabilized</t> with 0.1% Triton X-100 (Sigma) in <t>PBS</t> for 20 min. Nonspecific staining was blocked by incubation with 5% mouse serum in HBSS for 15 min. The cells on coverslips were then incubated with fluorescein isothiocyanate-conjugated anti-gC1 antibody (Syva Microtrak) (1:100 dilution), rinsed three times with HBSS, and mounted in mounting fluid (Syva Microtrak). Stained neurons were examined with a Bio-Rad MRC 600 confocal microscope. Note the similar distributions of gC antigen in the axon and cytoplasm in both micrographs. Bars, 40 μm (top) and 20 μm (bottom).
Thrombospondin 1 Tsp 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Immunotec inc tsp1 p10 antibody
Confocal micrographs of HSV-infected neurons stained for gC antigen at 15 hpi and after addition of human anti-gD monoclonal antibody (top) or control medium (bottom). The HSV inoculum (5 TCID50/cell) was aspirated after 1 h of incubation, and the cells were carefully washed once with HBSS. The HSV-infected or mock-infected dissociated neuronal cultures, incubated with a 1:2,500 dilution (400 ng/ml) of human anti-gD antibody, were fixed in 2.5% formaldehyde (ProSci Tech) in Sorensons buffer (pH 7.4) for 30 min <t>and</t> <t>permeabilized</t> with 0.1% Triton X-100 (Sigma) in <t>PBS</t> for 20 min. Nonspecific staining was blocked by incubation with 5% mouse serum in HBSS for 15 min. The cells on coverslips were then incubated with fluorescein isothiocyanate-conjugated anti-gC1 antibody (Syva Microtrak) (1:100 dilution), rinsed three times with HBSS, and mounted in mounting fluid (Syva Microtrak). Stained neurons were examined with a Bio-Rad MRC 600 confocal microscope. Note the similar distributions of gC antigen in the axon and cytoplasm in both micrographs. Bars, 40 μm (top) and 20 μm (bottom).
Tsp1 P10 Antibody, supplied by Immunotec inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Cambridge Isotope Laboratories sodium (3-trimethylsilyl)-2,2,3,3-tetradeuteriopropionate (tsp)
Confocal micrographs of HSV-infected neurons stained for gC antigen at 15 hpi and after addition of human anti-gD monoclonal antibody (top) or control medium (bottom). The HSV inoculum (5 TCID50/cell) was aspirated after 1 h of incubation, and the cells were carefully washed once with HBSS. The HSV-infected or mock-infected dissociated neuronal cultures, incubated with a 1:2,500 dilution (400 ng/ml) of human anti-gD antibody, were fixed in 2.5% formaldehyde (ProSci Tech) in Sorensons buffer (pH 7.4) for 30 min <t>and</t> <t>permeabilized</t> with 0.1% Triton X-100 (Sigma) in <t>PBS</t> for 20 min. Nonspecific staining was blocked by incubation with 5% mouse serum in HBSS for 15 min. The cells on coverslips were then incubated with fluorescein isothiocyanate-conjugated anti-gC1 antibody (Syva Microtrak) (1:100 dilution), rinsed three times with HBSS, and mounted in mounting fluid (Syva Microtrak). Stained neurons were examined with a Bio-Rad MRC 600 confocal microscope. Note the similar distributions of gC antigen in the axon and cytoplasm in both micrographs. Bars, 40 μm (top) and 20 μm (bottom).
Sodium (3 Trimethylsilyl) 2,2,3,3 Tetradeuteriopropionate (Tsp), supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Cambridge Isotope Laboratories phosphate buffer with trimethylsilylpropanoic acid (tsp)
Confocal micrographs of HSV-infected neurons stained for gC antigen at 15 hpi and after addition of human anti-gD monoclonal antibody (top) or control medium (bottom). The HSV inoculum (5 TCID50/cell) was aspirated after 1 h of incubation, and the cells were carefully washed once with HBSS. The HSV-infected or mock-infected dissociated neuronal cultures, incubated with a 1:2,500 dilution (400 ng/ml) of human anti-gD antibody, were fixed in 2.5% formaldehyde (ProSci Tech) in Sorensons buffer (pH 7.4) for 30 min <t>and</t> <t>permeabilized</t> with 0.1% Triton X-100 (Sigma) in <t>PBS</t> for 20 min. Nonspecific staining was blocked by incubation with 5% mouse serum in HBSS for 15 min. The cells on coverslips were then incubated with fluorescein isothiocyanate-conjugated anti-gC1 antibody (Syva Microtrak) (1:100 dilution), rinsed three times with HBSS, and mounted in mounting fluid (Syva Microtrak). Stained neurons were examined with a Bio-Rad MRC 600 confocal microscope. Note the similar distributions of gC antigen in the axon and cytoplasm in both micrographs. Bars, 40 μm (top) and 20 μm (bottom).
Phosphate Buffer With Trimethylsilylpropanoic Acid (Tsp), supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Cambridge Isotope Laboratories tsp (3-trimethylsilyl-2,2,3,3-tetradeutero sodium propionate)
Confocal micrographs of HSV-infected neurons stained for gC antigen at 15 hpi and after addition of human anti-gD monoclonal antibody (top) or control medium (bottom). The HSV inoculum (5 TCID50/cell) was aspirated after 1 h of incubation, and the cells were carefully washed once with HBSS. The HSV-infected or mock-infected dissociated neuronal cultures, incubated with a 1:2,500 dilution (400 ng/ml) of human anti-gD antibody, were fixed in 2.5% formaldehyde (ProSci Tech) in Sorensons buffer (pH 7.4) for 30 min <t>and</t> <t>permeabilized</t> with 0.1% Triton X-100 (Sigma) in <t>PBS</t> for 20 min. Nonspecific staining was blocked by incubation with 5% mouse serum in HBSS for 15 min. The cells on coverslips were then incubated with fluorescein isothiocyanate-conjugated anti-gC1 antibody (Syva Microtrak) (1:100 dilution), rinsed three times with HBSS, and mounted in mounting fluid (Syva Microtrak). Stained neurons were examined with a Bio-Rad MRC 600 confocal microscope. Note the similar distributions of gC antigen in the axon and cytoplasm in both micrographs. Bars, 40 μm (top) and 20 μm (bottom).
Tsp (3 Trimethylsilyl 2,2,3,3 Tetradeutero Sodium Propionate), supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
ICN Biomedicals tsp[d4]
Confocal micrographs of HSV-infected neurons stained for gC antigen at 15 hpi and after addition of human anti-gD monoclonal antibody (top) or control medium (bottom). The HSV inoculum (5 TCID50/cell) was aspirated after 1 h of incubation, and the cells were carefully washed once with HBSS. The HSV-infected or mock-infected dissociated neuronal cultures, incubated with a 1:2,500 dilution (400 ng/ml) of human anti-gD antibody, were fixed in 2.5% formaldehyde (ProSci Tech) in Sorensons buffer (pH 7.4) for 30 min <t>and</t> <t>permeabilized</t> with 0.1% Triton X-100 (Sigma) in <t>PBS</t> for 20 min. Nonspecific staining was blocked by incubation with 5% mouse serum in HBSS for 15 min. The cells on coverslips were then incubated with fluorescein isothiocyanate-conjugated anti-gC1 antibody (Syva Microtrak) (1:100 dilution), rinsed three times with HBSS, and mounted in mounting fluid (Syva Microtrak). Stained neurons were examined with a Bio-Rad MRC 600 confocal microscope. Note the similar distributions of gC antigen in the axon and cytoplasm in both micrographs. Bars, 40 μm (top) and 20 μm (bottom).
Tsp[D4], supplied by ICN Biomedicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbr+buffer/tsp+d4/us08148506-253-22-23
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90
Hilgenberg gmbh 70 μl d 2 o buffer (100% d 2 o, 500 mm nah 2 po 4 /na 2 hpo 4 , 0.1% tsp, ph 3.5)
Confocal micrographs of HSV-infected neurons stained for gC antigen at 15 hpi and after addition of human anti-gD monoclonal antibody (top) or control medium (bottom). The HSV inoculum (5 TCID50/cell) was aspirated after 1 h of incubation, and the cells were carefully washed once with HBSS. The HSV-infected or mock-infected dissociated neuronal cultures, incubated with a 1:2,500 dilution (400 ng/ml) of human anti-gD antibody, were fixed in 2.5% formaldehyde (ProSci Tech) in Sorensons buffer (pH 7.4) for 30 min <t>and</t> <t>permeabilized</t> with 0.1% Triton X-100 (Sigma) in <t>PBS</t> for 20 min. Nonspecific staining was blocked by incubation with 5% mouse serum in HBSS for 15 min. The cells on coverslips were then incubated with fluorescein isothiocyanate-conjugated anti-gC1 antibody (Syva Microtrak) (1:100 dilution), rinsed three times with HBSS, and mounted in mounting fluid (Syva Microtrak). Stained neurons were examined with a Bio-Rad MRC 600 confocal microscope. Note the similar distributions of gC antigen in the axon and cytoplasm in both micrographs. Bars, 40 μm (top) and 20 μm (bottom).
70 μl D 2 O Buffer (100% D 2 O, 500 Mm Nah 2 Po 4 /Na 2 Hpo 4 , 0.1% Tsp, Ph 3.5), supplied by Hilgenberg gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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70 μl d 2 o buffer (100% d 2 o, 500 mm nah 2 po 4 /na 2 hpo 4 , 0.1% tsp, ph 3.5) - by Bioz Stars, 2026-10
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90
CH Instruments chi-squared test
Confocal micrographs of HSV-infected neurons stained for gC antigen at 15 hpi and after addition of human anti-gD monoclonal antibody (top) or control medium (bottom). The HSV inoculum (5 TCID50/cell) was aspirated after 1 h of incubation, and the cells were carefully washed once with HBSS. The HSV-infected or mock-infected dissociated neuronal cultures, incubated with a 1:2,500 dilution (400 ng/ml) of human anti-gD antibody, were fixed in 2.5% formaldehyde (ProSci Tech) in Sorensons buffer (pH 7.4) for 30 min <t>and</t> <t>permeabilized</t> with 0.1% Triton X-100 (Sigma) in <t>PBS</t> for 20 min. Nonspecific staining was blocked by incubation with 5% mouse serum in HBSS for 15 min. The cells on coverslips were then incubated with fluorescein isothiocyanate-conjugated anti-gC1 antibody (Syva Microtrak) (1:100 dilution), rinsed three times with HBSS, and mounted in mounting fluid (Syva Microtrak). Stained neurons were examined with a Bio-Rad MRC 600 confocal microscope. Note the similar distributions of gC antigen in the axon and cytoplasm in both micrographs. Bars, 40 μm (top) and 20 μm (bottom).
Chi Squared Test, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Porphyrin Systems GbR 5,10,15,20-tetrakis(4-sulfonatophenyl)-porphine pd:tspp
Confocal micrographs of HSV-infected neurons stained for gC antigen at 15 hpi and after addition of human anti-gD monoclonal antibody (top) or control medium (bottom). The HSV inoculum (5 TCID50/cell) was aspirated after 1 h of incubation, and the cells were carefully washed once with HBSS. The HSV-infected or mock-infected dissociated neuronal cultures, incubated with a 1:2,500 dilution (400 ng/ml) of human anti-gD antibody, were fixed in 2.5% formaldehyde (ProSci Tech) in Sorensons buffer (pH 7.4) for 30 min <t>and</t> <t>permeabilized</t> with 0.1% Triton X-100 (Sigma) in <t>PBS</t> for 20 min. Nonspecific staining was blocked by incubation with 5% mouse serum in HBSS for 15 min. The cells on coverslips were then incubated with fluorescein isothiocyanate-conjugated anti-gC1 antibody (Syva Microtrak) (1:100 dilution), rinsed three times with HBSS, and mounted in mounting fluid (Syva Microtrak). Stained neurons were examined with a Bio-Rad MRC 600 confocal microscope. Note the similar distributions of gC antigen in the axon and cytoplasm in both micrographs. Bars, 40 μm (top) and 20 μm (bottom).
5,10,15,20 Tetrakis(4 Sulfonatophenyl) Porphine Pd:Tspp, supplied by Porphyrin Systems GbR, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Confocal micrographs of HSV-infected neurons stained for gC antigen at 15 hpi and after addition of human anti-gD monoclonal antibody (top) or control medium (bottom). The HSV inoculum (5 TCID50/cell) was aspirated after 1 h of incubation, and the cells were carefully washed once with HBSS. The HSV-infected or mock-infected dissociated neuronal cultures, incubated with a 1:2,500 dilution (400 ng/ml) of human anti-gD antibody, were fixed in 2.5% formaldehyde (ProSci Tech) in Sorensons buffer (pH 7.4) for 30 min and permeabilized with 0.1% Triton X-100 (Sigma) in PBS for 20 min. Nonspecific staining was blocked by incubation with 5% mouse serum in HBSS for 15 min. The cells on coverslips were then incubated with fluorescein isothiocyanate-conjugated anti-gC1 antibody (Syva Microtrak) (1:100 dilution), rinsed three times with HBSS, and mounted in mounting fluid (Syva Microtrak). Stained neurons were examined with a Bio-Rad MRC 600 confocal microscope. Note the similar distributions of gC antigen in the axon and cytoplasm in both micrographs. Bars, 40 μm (top) and 20 μm (bottom).

Journal:

Article Title: Neutralizing Antibodies Inhibit Axonal Spread of Herpes Simplex Virus Type 1 to Epidermal Cells In Vitro

doi:

Figure Lengend Snippet: Confocal micrographs of HSV-infected neurons stained for gC antigen at 15 hpi and after addition of human anti-gD monoclonal antibody (top) or control medium (bottom). The HSV inoculum (5 TCID50/cell) was aspirated after 1 h of incubation, and the cells were carefully washed once with HBSS. The HSV-infected or mock-infected dissociated neuronal cultures, incubated with a 1:2,500 dilution (400 ng/ml) of human anti-gD antibody, were fixed in 2.5% formaldehyde (ProSci Tech) in Sorensons buffer (pH 7.4) for 30 min and permeabilized with 0.1% Triton X-100 (Sigma) in PBS for 20 min. Nonspecific staining was blocked by incubation with 5% mouse serum in HBSS for 15 min. The cells on coverslips were then incubated with fluorescein isothiocyanate-conjugated anti-gC1 antibody (Syva Microtrak) (1:100 dilution), rinsed three times with HBSS, and mounted in mounting fluid (Syva Microtrak). Stained neurons were examined with a Bio-Rad MRC 600 confocal microscope. Note the similar distributions of gC antigen in the axon and cytoplasm in both micrographs. Bars, 40 μm (top) and 20 μm (bottom).

Article Snippet: The HSV-infected or mock-infected dissociated neuronal cultures, incubated with a 1:2,500 dilution (400 ng/ml) of human anti-gD antibody, were fixed in 2.5% formaldehyde (ProSci Tech) in Sorensons buffer (pH 7.4) for 30 min and permeabilized with 0.1% Triton X-100 (Sigma) in PBS for 20 min. Nonspecific staining was blocked by incubation with 5% mouse serum in HBSS for 15 min.

Techniques: Infection, Staining, Incubation, Microscopy